➥ This training program includes hardware and software introduction Outline of the HPLC training.
➥ Introduction to HPLC and basic principle of HPLC
➥ Brief introduction to manual injection valves, isocratic pumps and UV detection
➥ Make injection and run basic HPLC chromatogram.
➥ Effects of changing eluent composition, flow rate, column and detector • Making up an accurate mobile phase composition • Make flow path connections
➥ Basic precautions to avoid problems
➥ HPLC solvent selection
➥ Column: replacement, efficiency and capacity
➥ Introduction to chromatographic interpretation
➥ Introduction to sample preparation
➥ Introduction to autosamplers
➥ Safety: Working with small particle size materials, high pressures, UV radiation and electronic safety
➥ Peak identification
➥ Retention times and appropriate time windows for each peak